Blog articles
HPLC: What to do in case of peaks being too broad?
HPLC methods are often part of routine analysis of drugs or drug substances in pharmaceutical QC laboratories under GMP conditions.
The shape of the peak of a HPLC chromatogram is extremely critical for its evaluation. Subtle changes in the shape during a quantitative measurement can lead to misleading results and in turn can ruin the entire measurements obtained throughout the experiment. Therefore, it is important to keep a watchful eye on the “peak shape”.
Peak symmetry, asymmetry and their causes in HPLC
In another blog post, we have already discussed the problem of peak broadening under the topic "HPLC: What to do in case of peaks being too broad?” while focussing on symmetrical broad peaks. In this blog post, we will emphasize more on the asymmetric peaks.