Journal Club: Validation of a cIEF method for antibody identification
Introduction and background
Monoclonal antibodies (mABs) are monovalent immunoglobulin molecules that bind only to the same epitope. These antibodies have a wide range of applications such as in diagnostics, analytics, and as drugs in the treatment of cancer and other autoimmune diseases. The emerging market of mAB biosimilars demands proper characterization after previously performed isolation of these molecules. Isolating these antibodies however is quite a challenge and was first done by Georges Köhler and César Milstein, for which they were awarded the Nobel prize in medicine and physiology in the year 1984.